Study on the galactose oxidase biosensor based on SiO2 sol-gel

A galactose oxidase biosensor is prepared based on the Prussian Blue(PB) modified electrode combined with SiO₂ sol-gel immobilization method in a "sandwich configuration". Its electrochemical characterization and related experiment condition have been studied in detail. The optimin detection potential is -100mV at pH 7.0, 25 °C.Under selected condition the sensor offered high sensitivity of 0.127μA (mmol·L^-1)^-1, a linear range of 0-18 mmol·L^-1 and a detection limit of 0.03mmol·L^-1 ,The response time is 10s. Both stability and selectivity of the biosensor are ideal.