Bio-MEMS sensors for real-time shear stress on endothelial cell dynamics

Author

Hsiai, Tzung K. ; Cho, Sung K. ; Wong, Pak K. ; Ing, Michael ; Navab, Mohammad ; Reddy, Srinuvasa ; Demer, Linda L. ; Ho, Chih M.

Author_Institution

Dept. of Biomed. Eng., Univ. of Southern California, Los Angeles, CA, USA

Volume

3

fYear

2002

fDate

23-26 Oct. 2002

Firstpage

1828

Abstract

Precise characterization of shear stress in the arterial trees is critical to elucidate the effects of spatial versus temporal shear stress gradients on the biological activities of endothelial cells (EC). We developed micro electrical mechanical systems (MEMS) sensors, comparable to the size of EC (2 × 80 μm), to deliver the spatial and temporal resolution necessary at a frequency response > 100 Hz. We provided the first in vitro evidence of real-time wall shear stress on EC couple with real-time gene expression of monocyte chemoattractant protein (MCP-1).

Keywords

biological techniques; blood vessels; cellular biophysics; frequency response; haemorheology; microsensors; proteins; 100 Hz; 80 micron; arterial trees; bio-MEMS sensors; biological activities; cell morphology regulation; endothelial cell dynamics; monocytes; real-time gene expression; real-time shear stress; spatial shear stress gradients; temporal shear stress gradients; Biosensors; Cells (biology); Mechanical sensors; Mechanical systems; Micromechanical devices; Real time systems; Sensor phenomena and characterization; Sensor systems; Spatial resolution; Stress;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint

ISSN

1094-687X

Print_ISBN

0-7803-7612-9

Type

conf

DOI

10.1109/IEMBS.2002.1053047

Filename

1053047