On proper simulation of phenomena influencing image formation in fluorescence microscopy

Author

Svoboda, David ; Ulman, Vladimir ; Matyska, Ludek ; Maska, Martin ; Bella, Jan ; Stejskal, Stanislav

Author_Institution

Centre for Biomed. Image Anal., Masaryk Univ., Brno, Czech Republic

fYear

2014

fDate

27-30 Oct. 2014

Firstpage

3944

Lastpage

3948

Abstract

The simulation plays an important role in biomedical image analysis as it can inherently provide large collections of image data with absolute ground truth. Compared to real image data typically annotated by some expert, the computer generated data still lacks the authenticity due to the simplifications of various natural phenomena. In this paper, we focus on simulating the photobleaching effect and the uneven illumination, being simplified or even omitted from the majority of present simulation toolkits, to considerably improve the authenticity of computer generated data.

Keywords

biomedical optical imaging; fluorescence; medical image processing; optical saturable absorption; authenticity; biomedical image analysis; computer generated data; fluorescence microscopy; image formation; photobleaching effect; uneven illumination; Biological system modeling; Computational modeling; Computers; Data models; Lighting; Microscopy; Photobleaching; Biomedical imaging; Fluorescence microscopy; Photobleaching; Simulation; Uneven illumination;

fLanguage

English

Publisher

ieee

Conference_Titel

Image Processing (ICIP), 2014 IEEE International Conference on

Conference_Location

Paris

Type

conf

DOI

10.1109/ICIP.2014.7025801

Filename

7025801