Title :
On proper simulation of phenomena influencing image formation in fluorescence microscopy
Author :
Svoboda, David ; Ulman, Vladimir ; Matyska, Ludek ; Maska, Martin ; Bella, Jan ; Stejskal, Stanislav
Author_Institution :
Centre for Biomed. Image Anal., Masaryk Univ., Brno, Czech Republic
Abstract :
The simulation plays an important role in biomedical image analysis as it can inherently provide large collections of image data with absolute ground truth. Compared to real image data typically annotated by some expert, the computer generated data still lacks the authenticity due to the simplifications of various natural phenomena. In this paper, we focus on simulating the photobleaching effect and the uneven illumination, being simplified or even omitted from the majority of present simulation toolkits, to considerably improve the authenticity of computer generated data.
Keywords :
biomedical optical imaging; fluorescence; medical image processing; optical saturable absorption; authenticity; biomedical image analysis; computer generated data; fluorescence microscopy; image formation; photobleaching effect; uneven illumination; Biological system modeling; Computational modeling; Computers; Data models; Lighting; Microscopy; Photobleaching; Biomedical imaging; Fluorescence microscopy; Photobleaching; Simulation; Uneven illumination;
Conference_Titel :
Image Processing (ICIP), 2014 IEEE International Conference on
Conference_Location :
Paris
DOI :
10.1109/ICIP.2014.7025801
