Title :
Real-time Detection of Protein Interactions Using Pyrolyzed Carbon
Author :
Lee, Jung A ; Lee, Seung S. ; Lee, Kwang-Cheol ; Park, Se II
Author_Institution :
Korea Adv. Inst. of Sci. & Technol., Daejeon
Abstract :
We present label-free, real-time detection of streptavidin binding using the pyrolyzed carbon with a p-type conduction behavior in aqueous solution, which is functionalized with amine-conjugated biotin. Pyrolyzed carbon derived from AZ 5214E are fabricated by photolithography and photoresist thermal decomposition in a N2 atmosphere. The streptavidin-biotin interactions on the pyrolyzed carbon are detected successfully by current change after streptavidin binding.
Keywords :
photolithography; pyrolysis; amine-conjugated biotin; aqueous solution; p-type conduction behavior; photolithography; photoresist thermal decomposition; protein interactions; pyrolyzed carbon; real-time detection; streptavidin binding; streptavidin-biotin interactions; Atmosphere; Conductivity; Electrical resistance measurement; Electrodes; Fabrication; Gold; Lithography; Proteins; Resists; Temperature;
Conference_Titel :
Sensors, 2006. 5th IEEE Conference on
Conference_Location :
Daegu
Print_ISBN :
1-4244-0375-8
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2007.355546
