Title :
Investigation of cell localisation pattern in 3 dimensional micro-tissues
Author :
Ozturk, Sukru ; Aydin, Veli Kaan ; Karakuzu, Betul ; Sendemir-Urkmez, Aylin
Author_Institution :
Biyomedikal Teknolojiler Anabilim Dali, Ege Univ., Izmir, Turkey
Abstract :
It is impossible to mimic mammalian tissues by classical two-dimensional (2D) techniques. In this study, it is aimed to take advantage of self-assembly characteristics of cells in physiological conditions to achieve 3D tissues in vitro. Human Osteosarcoma cells (SaOS-2) were co-cultured with human umbilical vein endothelial cells (HUVEC) and human skin fibroblast cells (Detroit) were co-cultured with human skin keratinocyte cells (HS2) in agar gels that were formed using 3D Petri Dish® technique, and localization of cells were examined. Affect of cell number on localization was also investigated via using different cell ratios in co-culture systems. The change over time in the size of micro-tissues was determined using ImageJ program. The fluorescence microscope examination revealed that in SaOS-2 - HUVEC co-culture system, cells were localized randomly, and there wasn´t any significant effect of cell number on localization pattern. In HS2 - Detroit co-culture system, fibroblasts were localized in the core and the keratinocytes were found on the outer shell, and cell number didn´t affect this pattern. In microtissues formed by HS2 - Detroit co-culture, there was a significant increase in size after 72 hours. In the SaOS-2 - HUVEC co-culture, there was a significant reduction between the first and the fifth hours.
Keywords :
biomedical optical imaging; biomimetics; cellular biophysics; fluorescence; gels; self-assembly; skin; tissue engineering; 3D Petri dish technique; ImageJ program; agar gels; cell localisation pattern; cell self-assembly characteristics; fluorescence microscope examination; human osteosarcoma cell-2; human skin fibroblast cells; human skin keratinocyte cells; human umbilical vein endothelial cells; mammalian tissues; physiological conditions; three-dimensional microtissues; time 72 hour; Art; Fibroblasts; In vitro; In vivo; Self-assembly; Skin; Three-dimensional displays;
Conference_Titel :
Biomedical Engineering Meeting (BIYOMUT), 2014 18th National
Conference_Location :
Istanbul
DOI :
10.1109/BIYOMUT.2014.7026351