Title :
Lipase Catalyzed Synthesis of Ethyl Lactate
Author :
Huang Zhihong ; Gao Jing ; Zhao Tiantao ; Li Weijie ; Zhou Liya ; He Ying
Author_Institution :
Sch. of Chem. Eng. & Technol., Hebei Univ. of Technol., Tianjin, China
Abstract :
Ethyl lactate was synthesized by lipase catalyzed between lactic acid and ethanol in organic medium for the first time. The experiments showed that the optimal solvent is Tert-buty alcohol and the optimal lipase is Novozym 435. The optimal reaction with a conversion of 77% can be reached when the molar ratio (acid: alcohol) is 1:8, the temperature is 60degC, the acid concentration is 0.3 mol/L, the lipase concentration is 45 g/mol, reaction time is 24 hours and the revolutions is 200 rpm. Novozym 435 can be used continuously for six times and a conversion of 60% can be reached.
Keywords :
biochemistry; biotechnology; catalysis; enzymes; solvents (industrial); Novozym 435 lipase; ethanol reagent; ethyl lactate lipase catalysed synthesis; lactic acid reagent; organic medium; temperature 60 degC; tert-buty alcohol solvent; time 24 hour; Biochemistry; Chemical analysis; Chemical technology; Environmentally friendly manufacturing techniques; Ethanol; Molecular sieves; Pollution; Silicon compounds; Solvents; Temperature;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5163016
