A Microfluidic Device With Concave Surfaced Micropost Array for Rare Cell Capture

Author

Howard, Daniel J. ; Young-Jin Seo ; Bumsuk Hahm ; Kwon, Jae W.

Author_Institution

Dept. of Biol. Eng., Univ. of Missouri, Columbia, MO, USA

Volume

14

Issue

1

fYear

2014

fDate

Jan. 2014

Firstpage

5

Lastpage

6

Abstract

Microfluidic devices are becoming a standard method of lab analysis of many different types of samples. This letter presents a uniquely shaped micropost structure set in an arrayed fashion for sensing and cell capture purposes. After simulating the design to confirm its improved functionality, a model cell line was used to determine the capture yield and capture rate of the device. Specific capture was achieved by coating the array of microposts with antibodies that target the epithelial cell adhesion molecule, a common biomarker for many types of metastatic cancer.

Keywords

adhesion; bioMEMS; cancer; cellular biophysics; coatings; microfluidics; molecular biophysics; proteins; antibodies; biomarker; capture rate; capture yield; cell capture purposes; coating; concave surfaced micropost array; epithelial cell adhesion molecule; lab analysis; metastatic cancer; microfluidic device; model cell line; rare cell capture; sensing purposes; uniquely shaped micropost structure set; Arrays; Cancer; Educational institutions; Fabrication; Microfluidics; Standards; Tumors; Concave micropost; capture rate; cell capture; microfluidics;

fLanguage

English

Journal_Title

Sensors Journal, IEEE

Publisher

ieee

ISSN

1530-437X

Type

jour

DOI

10.1109/JSEN.2013.2283066

Filename

6609066