Study on Remove of Sulfide from Petroleum Refinery by Immobilized Thiobacillus ferrooxidans in Packed Bed Bioreactor

In order to remove sulfide from petroleum refinery, An packed bed bioreactor was constructed by immobilizing Thiobacillus ferrooxidans (T. f) on polyurethane foam HG-12 as carrier. Under the optimum condition of the immobilization bioreactor: air flow rate 200 L/h, 30degC and pH 2.0, a maximum Fe²⁺ oxidation of 7.21g/L.h was attained. And the complex compound forming on the surface of the carrier in the operation of bioreactor was identified as jarosite by X-ray diffraction analysis. In the packed bed bioreactor, the removal of sulfide was higher than 99.47%.