Title :
The Adsorption of Protein Molecules at a Crystal/solution Interface Observed by an Improved TIRFM
Author :
Dai, Guoliang ; Sazaki, Gen
Author_Institution :
Nat. Microgravity Lab., Chinese Acad. of Sci., Beijing, China
Abstract :
We have improved the ordinary total internal reflection fluorescence microscopy (TIRFM). Two improvements have been achieved, one is the interface between opaque material and solution can be observed, another is the interface far away (usually several ten micro meters) the objective lens can be observed. By this improved TIRFM, the adsorption of protein molecules at a crystal/solution interface had been successfully observed. We have obtained the results of relationship between the amount of adsorbed protein molecules on bunched steps and the height of bunched steps of a protein crystal.
Keywords :
adsorption; bio-optics; biological techniques; fluorescence; molecular biophysics; optical microscopy; proteins; TIRFM; crystal-solution interface; objective lens; opaque material; protein molecule adsorption; total internal reflection fluorescence microscopy; Chemicals; Crystalline materials; Fluorescence; Laser beams; Lenses; Microscopy; Optical materials; Optical reflection; Proteins; Solids;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5163569
