Title :
Fully integraed droplet based point-of-care platform for molecular detection from crude biosamples
Author :
Zhang, Yi ; Park, Seungkyung ; Yang, Samuel ; Wang, Tza-Huei
Author_Institution :
Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA
Abstract :
This paper reports a droplet microfluidic, sample-to-answer platform for the detection of disease biomarkers and infectious pathogens using crude biosamples. The platform exploited the dual functionality of silica superparamagnetic particles (SSP) for solid phase extraction of DNA and magnetic actuation. This enabled the integration of sample preparation and genetic analysis within discrete droplets, including the steps of cell lysis, DNA binding, washing, elution, amplification and detection. The microfluidic device was self contained, with all reagents stored in droplets, thereby eliminating the need for fluidic coupling to external reagent reservoirs. The device incorporated unique surface topographic features to assist droplet manipulation. Pairs of micro-elevations are created to form slits that facilitate efficient splitting of SSP from droplets. In addition, a compact sample handling stage, which integrated the magnet manipulator, the droplet microfluidic device and a Peltier thermal cycler, was built for convenient droplet manipulation and real-time detection. Feasibility of the platform was demonstrated by analysing ovarian cancer biomarker Rsf-1 and detecting Escherichia coli with the real time polymerase chain reaction and the real time helicase dependent amplification.
Keywords :
DNA; Peltier effect; bioMEMS; biochemistry; biosensors; cancer; cellular biophysics; drops; enzymes; genetics; gynaecology; microfluidics; microorganisms; microsensors; molecular biophysics; surface topography; DNA binding; Escherichia coli; Peltier thermal cycler; amplification; cell lysis; compact sample handling stage; crude biosamples; disease biomarkers; droplet microfluidic; elution; fluidic coupling; genetic analysis; infectious pathogens; magnet manipulator; magnetic actuation; molecular detection; ovarian cancer biomarker Rsf-1; point-of-care platform; real time helicase dependent amplification; real time polymerase chain reaction; real-time detection; sample-to-answer platform; silica superparamagnetic particles; solid phase extraction; surface topographic features; washing; Biomarkers; Cancer; DNA; Fluorescence; Microfluidics; Real time systems; Surface topography; µTAS; Droplet; PCR; melting curve analysis; point of care; real time; sample preparation;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International
Conference_Location :
Beijing
Print_ISBN :
978-1-4577-0157-3
DOI :
10.1109/TRANSDUCERS.2011.5969368