Title :
A platform for in situ real-time measurement of protein induced conformational changes of DNA
Author :
Spuhler, Philipp S. ; Knezevic, Jelena ; Yalcin, Ayca ; Dröge, Peter ; Rant, Ulrich ; Ünlü, M. Selim
Author_Institution :
Biomed. Eng., Boston Univ., Boston, MA, USA
Abstract :
A platform for in situ and real-time measurement of protein induced conformational changes in dsDNA is presented. We demonstrate protein induced bending upon sequence specific binding of integration host factor (IHF) to dsDNA probes.
Keywords :
DNA; bioMEMS; biosensors; lab-on-a-chip; molecular biophysics; molecular configurations; proteins; bending; dsDNA; dsDNA probes; in situ real-time measurement; integration host factor; protein induced-conformational changes; sequence specific binding; Biomedical measurements; DNA; Electric variables measurement; Electrodes; Fluorescence; Gold; Probes; Proteins; Sequences; Throughput;
Conference_Titel :
LEOS Annual Meeting Conference Proceedings, 2009. LEOS '09. IEEE
Conference_Location :
Belek-Antalya
Print_ISBN :
978-1-4244-3680-4
Electronic_ISBN :
1092-8081
DOI :
10.1109/LEOS.2009.5343182
