A miniaturized optically-induced fluorescence-activated cell sorter

We integrate the concept of liquid light-driven approach and a cell fluorescent detection system to sort different labeled color HepG2 and 3T3 cells. The optoelectroosmosis flow (OEOF) method would drive the liquid and reverse the flow direction immediately only with light pattern movement. A dielectrophoresis (DEP) force is applied to focal the moving cells as a line. A LabVIEW program would detect the fluorescent cell color and switch the projected light pattern automatically to manipulate the mobile cell real-time. After the OEOF operation, the selected cell is able to push a lateral displacement with 120μm distance against original direction. Based on these four features, this article demonstrates a potential for cells sorting with light driving approaches.