Title :
The development of an ova holding device made of microporous glass plate for genetic engineering
Author :
Fujisato, T. ; Abe, S. ; Tsuji, T. ; Sada, M. ; Miyawaki, F. ; Ohba, K.
Author_Institution :
Nat. Cardiovascular Center Res. Inst., Osaka, Japan
fDate :
29 Oct-1 Nov 1998
Abstract :
In the conventional microinjection method using a micropipette, ovum manipulation is dependent upon the individual skills of the operators. In this study, the authors developed a newly invented ova holding device made of a microporous glass (MPG) disc which has a matrix of 5×5 micropockets with diameter of 130 μm. Ova are drawn into the micropockets following the circulation of solution across the MPG disc
Keywords :
biological techniques; cellular biophysics; genetics; glass; 130 mum; conventional microinjection method; genetic engineering; micropipette; micropockets matrix; ova holding device development; ovum manipulation; solution circulation; Animal structures; Cardiology; Control systems; Etching; Fluid flow; Genetic engineering; Glass manufacturing; Microinjection; Microscopy; Steel;
Conference_Titel :
Engineering in Medicine and Biology Society, 1998. Proceedings of the 20th Annual International Conference of the IEEE
Conference_Location :
Hong Kong
Print_ISBN :
0-7803-5164-9
DOI :
10.1109/IEMBS.1998.746116
