Title :
An acoustic plate mode study of complementary and non-complementary antigen/antibody reactions
Author :
Dahint, R. ; Bender, F. ; Grunze, M.
Author_Institution :
Angewandte Phys. Chem., Heidelberg Univ., Germany
Abstract :
Acoustic plate mode sensors have been used to monitor immunochemical reactions as a function of antigen concentration. In the studies, antibodies were covalently linked to the gold-coated sensing surface via mercaptoethanol, aminosilane and glutaraldehyde. Two antigen/antibody model systems have been used which differ in their ability to mutually bind one another. For sensor operation at about 150 MHZ a detection limit of about 0.5 μg/ml was obtained in both cases. No significant difference between the two systems was found for the value of the binding constants. They amount to about 1.108 1/mol and fall well into the range of binding constants reported for homogeneous immunoassays. A comparison of the sensor response obtained for the two model systems shows that about 70% of the immobilized antibodies are active
Keywords :
biosensors; macromolecules; molecular biophysics; proteins; surface acoustic wave sensors; 150 MHz; Au; Au coated sensing surface; acoustic plate mode; aminosilane; antigen concentration; antigen/antibody model systems; binding constants; biosensor; complementary antigen/antibody reactions; glutaraldehyde; homogeneous immunoassays; immobilized antibodies; immunochemical reactions; mercaptoethanol; non-complementary antigen/antibody reactions; Acoustic devices; Acoustic sensors; Biosensors; Chemical sensors; Ethanol; Immune system; Mechanical sensors; Monitoring; Proteins; Surface acoustic waves;
Conference_Titel :
Frequency Control Symposium, 1997., Proceedings of the 1997 IEEE International
Conference_Location :
Orlando, FL
Print_ISBN :
0-7803-3728-X
DOI :
10.1109/FREQ.1997.638515