Comparing Terahertz transmission response on pH-dependent apomyoglobin proteins dynamics with circular dichroism

Author

Junyi Qiu ; Bin Yang ; Sushko, Oleksandr ; Pikersgill, Richard W. ; Donnan, Robert S.

Author_Institution

Sch. of Electron. Eng. & Comput. Sci., Queen Mary, Univ. of London, London, UK

fYear

2014

fDate

8-10 Dec. 2014

Firstpage

1

Lastpage

3

Abstract

Terahertz time domain spectroscopy (THz-TDS) was used to study the transmission responses of pH-dependent apomyoglobin (ApoMb) dissolved solutions in 0.2-2.2 THz frequency domain, the THz-TDS technique was also benchmarked against circular dichroism (CD) by studying pH-related folding states changes of ApoMb protein. Results revealed that differences of pH-dependent ApoMb/water dynamics can be detected directly by the THz refractive index spectrum, and these differences are further proved to be caused mainly the effect of protonation of water and possibly water response leaded by protein conformation change.

Keywords

association; circular dichroism; molecular biophysics; molecular configurations; molecular dynamics method; proteins; refractive index; terahertz spectroscopy; ApoMb protein; THz refractive index spectrum; THz-TDS technique; circular dichroism; frequency 0.2 THz to 2.2 THz; pH-dependent apomyoglobin; pH-related folding states; protein conformation change; proteins dynamics; terahertz time domain spectroscopy; terahertz transmission response; water protonation; Absorption; Chemicals; Educational institutions; Market research; Proteins; Refractive index; Spectroscopy; Circular dichroism; THz-TDS; protein dynamics; refractive index;

fLanguage

English

Publisher

ieee

Conference_Titel

RF and Wireless Technologies for Biomedical and Healthcare Applications (IMWS-Bio), 2014 IEEE MTT-S International Microwave Workshop Series on

Conference_Location

London

Print_ISBN

978-1-4799-5445-2

Type

conf

DOI

10.1109/IMWS-BIO.2014.7032420

Filename

7032420