Analysis of cellular membrane changing induced by influenza virus infection

Influenza virus infection induced various changing of the host cell. At the beginning of influenza virus infection, the transcription from viral genome RNA by RNA-dependent RNA polymerase took place by cleavage of host capped RNA using own endonuclease for priming of mRNA synthesis. After the viral protein NS1 expression, NS1 binds to CPSF lead to inhibit polyA addition of host mRNA (1). Finally the influenza virus infection reduced host cell protein expression. While after viral genome transcription, viral membrane protein HA, NA and M1 expressed and localized on the cellular membrane. It is thought that this phenomenon induced the cellular membrane stiffness. We performed to measure the stiffness of cellular membrane using optical laser and collagen coated-beads. Interestingly at 4 hrs after influenza virus infection, the membrane stiffness changed and also the level of integrin in the cellular membrane was decreased.