Title :
FROG EGG-Array device integrated with fluidic channel and microelectrodes for chemical sensing
Author :
Tomida, Mitsuyoshi ; Murakami, Yasutaka ; Misawa, N.
Author_Institution :
Dept. of Electr. & Electron. Inf. Eng., Toyohashi Univ. of Technol., Toyohashi, Japan
Abstract :
This paper describes a membrane protein-based chemical sensor that is consisted of fluidic channels and cells (Xenopus laevis oocytes) expressing chemical receptors. The fluidic device has Si-based microfabricated electrodes to measure the Xenopus oocyte´s response to chemicals using two-electrode voltage clamping. The fluidic device was fabricated by combining fluidic channel made of acrylic resin and the electrode substrate. After the cell installing, the fluidic device can be separated to each fluidic channel that can measure an individual oocyte membrane potential change. We succeeded to array each oocyte in the device and to detect an individual Xenopus oocyte´s responses to chemical stimulus.
Keywords :
bioelectric potentials; biomembranes; biosensors; cellular biophysics; chemical sensors; microchannel flow; microelectrodes; microfabrication; microsensors; molecular biophysics; proteins; sensor arrays; Si; Si-based microfabricated electrodes; Xenopus laevis oocytes; acrylic resin; ce ll installing; chemical receptors; chemical sensing; chemical stimulus; electrode substrate; fluidic channel; frog egg-array device; membrane chemical sensor; microelectrodes; oocyte membrane potential; two-electrode voltage clamping; Biomembranes; Charge carrier processes; Chemical and biological sensors; Chemicals; Electrodes; Robot sensing systems; Substrates;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2014 IEEE 27th International Conference on
Conference_Location :
San Francisco, CA
DOI :
10.1109/MEMSYS.2014.6765640