Title :
Synthesis of (R)-(-)-Mandelic Acid Methyl Ester by Asymmetric Reduction of Methyl Benzoylformate with Yeast Cells
Author :
Zhimin, Ou ; Yong, Liu ; Yinkang, Nan
Author_Institution :
Pharmaceuticals Coll., Zhejiang Univ. of Technol., Hang Zhou, China
Abstract :
An efficient yeast cell biotransformation process was set up for asymmetric synthesis of (R)-(-)-mandelic acid methyl ester, a key drug intermediate. Saccharomyces cerevisiae 21 was selected as optimum strain for biotransformation. The optimum reduction conditions are as follows: substrate concentration 22 g/L, cell concentration 150 g/L, reaction time 36 h, 30°C, pH5.0. Conversion and enantiometric excess of (R)-(-)-mandelic acid methyl ester reached 99.4% and 99.9%.
Keywords :
biotechnology; drugs; microorganisms; R-(-)-mandelic acid methyl ester; Saccharomyces cerevisiae 21strain; asymmetric synthesis; cell concentration; drug intermediate; enantiometric excess; methyl benzoylformate; methyl ester synthesis; pH; reaction time; substrate concentration; time 36 h; yeast cell biotransformation process; Biochemistry; Biomass; Drugs; Educational institutions; Production; Strain; Substrates; (R)-(-)-mandelic acid methyl ester; asymmetric reduction; biotransformation; methyl benzoylformate;
Conference_Titel :
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location :
Macau, Macao
Print_ISBN :
978-1-4577-1987-5
DOI :
10.1109/iCBEB.2012.390
