DocumentCode :
2711764
Title :
A novel bioreactor for the dynamic flexural stimulation of tissue engineered heart valve biomaterials
Author :
Engelmayr, George ; Sutherland, Fraser W H ; Mayer, John E., Jr. ; Sacks, Michael S.
Author_Institution :
Dept. of Bioeng., Pittsburgh Univ., PA, USA
Volume :
1
fYear :
2002
fDate :
2002
Abstract :
A novel bioreactor was developed for the purpose of studying the effect of dynamic flexural stimulation on the properties of tissue engineered heart valve (TEHV) scaffolds and constructs. While pulse duplicator and flow loop bioreactors have shown promise in the development of functional tissue engineered cardiovascular constructs, these devices present several drawbacks when applied to the study of fundamental biomechanical phenomena, including: small sample capacity, anatomical sample geometry, and coupled mechanical stimuli. In contrast, our bioreactor was designed to provide a simple, user-controllable mode of mechanical stimulation; cyclic three-point bending; offer a sufficient sample capacity for statistically significant comparisons at multiple time points, and accommodate a simple sample geometry amenable to mechanical testing. The bioreactor has the capacity to dynamically flex twelve rectangular samples (2.5 × 0.75 × 0.2 cm) under sterile conditions in a humidified cell culture incubator operating at 37°C and 5 % CO2.
Keywords :
biomechanics; biomedical equipment; biomedical materials; cardiology; mechanical testing; 0.2 to 2.5 cm; 37 C; CO2; anatomical sample geometry; bioreactor; constructs; coupled mechanical stimuli; dynamic flexural stimulation; fundamental biomechanical phenomena; humidified cell culture incubator; rectangular samples; scaffolds; simple user-controllable mode mechanical stimulation mode; small sample capacity; sterile conditions; tissue engineered heart valve biomaterials; Biomedical engineering; Bioreactors; Cardiology; Electronics packaging; Geometry; Heart valves; Hospitals; Pediatrics; Surgery; Testing;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
ISSN :
1094-687X
Print_ISBN :
0-7803-7612-9
Type :
conf
DOI :
10.1109/IEMBS.2002.1137109
Filename :
1137109
Link To Document :
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