Non-destructive real-time imaging of cell morphology for tissue-engineering applications

Author

Hofmann, Matthias C. ; Whited, Bryce M. ; Goldstein, Aaron S. ; Freeman, Joseph W. ; Rylander, Marissa Nichole ; Rylander, Chris ; Furth, Mark ; Wang, Ge ; Soker, Shay ; Xu, Yong

Author_Institution

Bradley Dept. of Electr. & Comput. Eng., Virginia Polytech. Inst. & State Univ., Blacksburg, VA, USA

fYear

2011

fDate

19-24 June 2011

Firstpage

1

Lastpage

3

Abstract

A major barrier for progress in regenerative medicine is the inability to observe the process of tissue regeneration non-destructively, in real time, and with cellular level resolution. In order to overcome this difficult challenge, we have proposed and developed an imaging-bioreactor system based on fiber micro-devices. Our system is based on directly incorporating multiple hollow core fibers (HCFs) into a biocompatible tissue scaffold. By inserting fiber-based micro-mirrors into the HCFs and applying a straightforward scanning-reconstruction algorithm, we have demonstrated an imaging resolution of ~200 μm through an optically opaque scaffold.

Keywords

biomedical optical imaging; bioreactors; cellular biophysics; image reconstruction; image resolution; medical image processing; micromirrors; optical fibres; tissue engineering; biocompatible tissue scaffold; cell morphology; cellular level resolution; fiber microdevices; fiber-based micromirrors; imaging-bioreactor system; multiple hollow core fibers; nondestructive real-time imaging; optically opaque scaffold; regenerative medicine; straightforward scanning-reconstruction algorithm; tissue regeneration; tissue-engineering; Biomedical imaging; Fluorescence; Microwave integrated circuits; Optical fibers; Tissue engineering; cells; scaffold; tissue engineering;

fLanguage

English

Publisher

ieee

Conference_Titel

Information Optics (WIO), 2011 10th Euro-American Workshop on

Conference_Location

Benicassim

Print_ISBN

978-1-4577-1227-2

Electronic_ISBN

978-1-4577-1225-8

Type

conf

DOI

10.1109/WIO.2011.5981465

Filename

5981465