A digitized acquisition system for electroretinograms and its use in studying the effects of organic buffers on the isolated rat retina

Author

Finelli, Cynthia J. ; Doty, Sandra L. ; Svinarich, Kathryn A. ; Winkler, Barry S.

Author_Institution

GMI Eng. & Manage. Inst., Flint, MI, USA

Volume

5

fYear

1997

fDate

1997

Firstpage

2212

Abstract

A digitized acquisition system for recording the electroretinogram (ERG) of an isolated rat retina is presented. The system is used to study the effects of three distinct buffering agents (Trizma alone, Hepes alone, and a combination of prebubbled bicarbonate with Hepes) on the viability of the ERG. Amplitude of the a-wave, its decay over time, and absence or presence of the b-wave were monitored. Preliminary results indicate that bicarbonate is a necessary ingredient in the buffer system for long term viability of the retina

Keywords

analogue-digital conversion; data acquisition; electroretinography; medical signal processing; smoothing methods; ERG viability; Hepes; Trizma; a-wave amplitude; b-wave absence/presence; computer acquisition; data smoothing; decay over time; digitized acquisition system; electroretinograms; isolated rat retina; noise spike removal; organic buffers effect; perfusion medium; prebubbled bicarbonate; transient retinal action potential; Biomedical monitoring; Blood; Delay; Electrodes; Engineering management; Photoreceptors; Pigmentation; Retina; Shape; Working environment noise;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1997. Proceedings of the 19th Annual International Conference of the IEEE

Conference_Location

Chicago, IL

ISSN

1094-687X

Print_ISBN

0-7803-4262-3

Type

conf

DOI

10.1109/IEMBS.1997.758797

Filename

758797