Integration of RSM model for optimization of immobilized lipase mediated solvent-free synthesis of flavour ester by genetic algorithm

Author

Garlapati, Vijay Kumar ; Vundavilli, Pandu Ranga ; Banerjee, Rintu

Author_Institution

Dept. of Biotechnol. & Bioinf., Jaypee Univ. of Inf. Technol., Waknaghat, India

fYear

2011

fDate

3-5 Nov. 2011

Firstpage

1

Lastpage

4

Abstract

In the present study, optimization of immobilized lipase mediated solvent-free synthesis of octyl acetate by transesterification has been carried out based on the experimentally validated RSM model. The effect of tansesterification variables namely alcohol molarity, reaction time, temperature, Immobilized lipase amount on molar conversion (%) was investigated. A maximum molar conversion of 92.92% was obtained with the GA parameters of tournament selection of size two, uniform crossover probability (P_c) of 0.5, bit-wise mutational probability (P_m) of 0.0029, population size of 80 and maximum number of generations of 382. The optimal values of the transesterification variables were found to be 2.09 M octanol in vinyl acetate, 12.61 h, 32°C and 26.01 U for alcohol molarity, reaction time, temperature and amount of Immobilized enzyme respectively.

Keywords

chemical engineering; chemical reactions; chemical technology; enzymes; genetic algorithms; response surface methodology; RSM model; alcohol molarity; bit-wise mutational probability; flavour ester; genetic algorithm; immobilized enzyme; immobilized lipase amount; immobilized lipase mediated solvent-free synthesis; molar conversion; octyl acetate; reaction time; tansesterification variable; temperature 32 degC; time 12.61 h; transesterification; transesterification temperature; uniform crossover probability; vinyl acetate; Biochemistry; Biological system modeling; Genetic algorithms; Information processing; Optimization; Production; USA Councils; Flavour ester; Genetic algorithm; Immobilized Lipase; Optimization; Solvent-free;

fLanguage

English

Publisher

ieee

Conference_Titel

Image Information Processing (ICIIP), 2011 International Conference on

Conference_Location

Himachal Pradesh

Print_ISBN

978-1-61284-859-4

Type

conf

DOI

10.1109/ICIIP.2011.6108936

Filename

6108936