Title :
Optical detection of molecular beacons in microfluidic devices
Author :
Balberg, Michal ; Hristova, Krassimira ; Brady, David J. ; Beebe, David J. ; Raskin, L.
Author_Institution :
Beckman Inst. for Adv. Sci. & Technol., Illinois Univ., Urbana, IL, USA
Abstract :
Hybridization and detection of E. coli ribosomal RNA with molecular beacons labeled with a fluorescent dye is achieved in a microfluidic device. The device consists of a four ports mixing chamber, where the probes and the target molecules mix by diffusion. The weak fluorescent signal is detected by two large core optical fibers that are placed on both sides of the channel and collect the light emitted in both directions. The fibers are coupled to a spectrophotometer. The detection limit for the system is 0.2 fmol
Keywords :
biological techniques; fibre optic sensors; fluidic devices; fluorescence; microfluidics; microorganisms; molecular biophysics; spectrophotometry; 4-ports mixing chamber; E. coli ribosomal RNA; fluorescent dye; hybridization; large core optical fibers; microfluidic devices; molecular beacons; optical detection; spectrophotometer; system detection limit; target molecules; Fluorescence; Microfluidics; Optical detectors; Optical devices; Optical fiber devices; Optical fibers; Optical mixing; Probes; RNA; Signal detection;
Conference_Titel :
Microtechnologies in Medicine and Biology, 1st Annual International, Conference On. 2000
Conference_Location :
Lyon
Print_ISBN :
0-7803-6603-4
DOI :
10.1109/MMB.2000.893820
