Title :
Photothermal nanoblade for single cell surgery
Author :
Wu, T.-H. ; Teslaa, T. ; Teitell, M.A. ; Chiou, P.-Y.
Author_Institution :
Dept. of Mech. & Aerosp. Eng., Univ. of California at Los Angeles, Los Angeles, CA, USA
Abstract :
We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest short laser pulse energy and convert it into a highly localized and specifically shaped explosive vapor bubble. Rapid bubble expansion and collapse punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. The membrane cutting pattern is controlled by the metallic nanostructure configuration, laser pulse polarization, and energy. Highly controllable, sub-micron sized circular hole pairs to half moon-like, or cat-door shaped, membrane cuts were realized in glutaraldehyde treated HeLa cells.
Keywords :
biomembranes; bubbles; cellular biophysics; nanobiotechnology; photothermal effects; surgery; bubble collapse; bubble expansion; cell membrane; explosive vapor bubble; glutaraldehyde treated HeLa cell; high speed fluidic flow; laser pulse polarization; metallic nanostructure; photothermal nanoblade; short laser pulse energy harvesting; single cell surgery; transient shear stress; Biomembranes; Heating; Laser beam cutting; Laser excitation; Optical polarization; Optical pulses; Ring lasers;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on
Conference_Location :
Cancun
Print_ISBN :
978-1-4244-9632-7
DOI :
10.1109/MEMSYS.2011.5734610
