Title :
A 96-well SPRI reactor in a photo-activated polycarbonate (PPC) microfluidic chip
Author :
Park, Daniel S. ; Hupert, Mateusz L. ; Witek, Makgorzata ; Guy, Jason ; Datta, Proyag ; You, Byoung H. ; Soper, Steven A. ; Murphy, Michael C.
Author_Institution :
Louisiana State Univ., University
Abstract :
A 96-well solid-phase reversible immobilization (SPRI) reactor in a micro molded, photo-activated polycarbonate (PPC) chip was designed, fabricated, and used to purify genomic DNA (gDNA) in a highly parallelized fashion. A SPRI reactor was designed to fit in a 96-well microtiter plate format with compatibility to the existing samples/reagents loading technologies. A high quality, metallic, large area mold insert (LAMI) was fabricated using a SU-8 based UV-LIGA technique. Micro molding of polycarbonate (PC) was done using hot embossing, resulting in good replication fidelity over the large surface area. The 96-well PPC-SPRI reactor, assembled by thermal fusion bonding, showed 100% efficiency for simple and fast purification of gDNA from whole cell lysates of bacterial species. The 96- well PPC-SPRI reactor will open up new avenues for low cost, disposable DNA sample purification in high throughput applications.
Keywords :
DNA; biomedical equipment; embossing; genetics; microfluidics; moulding; DNA sample purification; SU-8; UV-LIGA technique; genomic DNA purification; hot embossing; large area mold insert; micro molded chip; microfluidic chip; microtiter plate; photo-activated polycarbonate; solid-phase reversible immobilization reactor; Assembly; Bioinformatics; Bonding; DNA; Embossing; Genomics; Inductors; Microfluidics; Microorganisms; Purification;
Conference_Titel :
Micro Electro Mechanical Systems, 2007. MEMS. IEEE 20th International Conference on
Conference_Location :
Hyogo
Print_ISBN :
978-1-4244-095-5
Electronic_ISBN :
1084-6999
DOI :
10.1109/MEMSYS.2007.4432983
