DocumentCode :
2871334
Title :
A microfluidic device for electrofusion of biological membranes
Author :
Tresset, G. ; Takeuchi, Shoji
Author_Institution :
LIMMS, Tokyo Univ., Japan
fYear :
2004
fDate :
2004
Firstpage :
25
Lastpage :
28
Abstract :
This paper reports a microfabricated device with high aspect-ratio electrodes and low power consumption for the electrofusion of liposomes and cells. The applications may range from gene transfection or cell tracking to biophysical studies of membrane proteins. The device consists of 250 μm thick silicon electrodes bonded to a glass substrate and covered by a PDMS-coated glass slide. Liposomes were first aligned by AC voltage at 300 kHz and then fused with short DC pulses. The fusion yield can reach up 75% and is globally better for liposome diameters larger than 10 μm. The encapsulation of microbeads inside liposomes has been also demonstrated and opens up the route towards fusion-based delivery of artificial microstructures into cells.
Keywords :
biomembranes; biomolecular electronics; cellular biophysics; elemental semiconductors; genetics; microelectrodes; microfluidics; molecular biophysics; proteins; silicon; 10 micron; 250 micron; 300 kHz; Si; biological membranes; cell tracking; electrofusion; gene transfection; glass substrate; liposomes; membrane proteins; microfabricated device; microfluidic device; power consumption; silicon electrodes; Biomembranes; Bonding; Electrodes; Encapsulation; Energy consumption; Glass; Microfluidics; Proteins; Silicon; Voltage;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Micro Electro Mechanical Systems, 2004. 17th IEEE International Conference on. (MEMS)
Print_ISBN :
0-7803-8265-X
Type :
conf
DOI :
10.1109/MEMS.2004.1290513
Filename :
1290513
Link To Document :
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