Title :
A quadratic programming approach for the mosaicing of virtual slides that incorporates the positioning accuracy of the microscope stage
Author :
Steckhan, Dirk ; Paulus, Dietrich
Author_Institution :
Image Process. & Med. Eng. Dept., Fraunhofer-Inst. for Integrated Circuits IIS, Erlangen, Germany
fDate :
Aug. 31 2010-Sept. 4 2010
Abstract :
We describe a novel approach for creating virtual slides that incorporates the positioning accuracy of the microscope stage in the optimization step. To capture a complete slide in microscopy, a large number of fields of view have to be acquired by moving the microscope stage in a controlled way. These fields of view are aligned in such a way that a globally consistent virtual slide is formed. However, depending on the positioning repeatability of the stage and the accuracy of the stage calibration, this results in alignment errors. These errors are usually resolved by applying a mosaicing algorithm. Our algorithm extends known mosaicing approaches by analyzing the positioning accuracy of the stage and incorporating this knowledge to make the mosaicing process more robust.
Keywords :
medical image processing; optical microscopy; optimisation; microscope stage; mosaicing algorithm; mosaicing process; optimization; positioning repeatability; quadratic programming approach; stage calibration; Accuracy; Correlation; Equations; Microscopy; Optimization; Probes; Transforms; Algorithms; Blood Cells; Calibration; Cervix Uteri; Female; Histology; Humans; Image Processing, Computer-Assisted; Least-Squares Analysis; Microscopy; Reproducibility of Results; Skin;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2010 Annual International Conference of the IEEE
Conference_Location :
Buenos Aires
Print_ISBN :
978-1-4244-4123-5
DOI :
10.1109/IEMBS.2010.5626241