Title :
NADH-fluorometric biochemical gas sensor (bio-sniffer) for assessment of indoor air quality
Author :
Kudo, Hiroyuki ; Yamashita, Takayoshi ; Ming Ye ; Miyajima, Kiyotomi ; Arakawa, Takeshi ; Mitsubayashi, Kohji ; Gessei, Tomoko
Author_Institution :
Inst. of Biomater. & Bioeng., Tokyo Med. & Dental Univ., Tokyo, Japan
Abstract :
A fiber-optic biochemical gas sensor (bio-sniffer) for assessment of indoor formaldehyde was fabricated and tested. The bio-sniffer measures formaldehyde vapor as fluorescence of reduced nicotinamide adenine dinucleotide (NADH), which is the product of formaldehyde dehydrogenase (FALDH) reaction. Usually, an enzyme loses its specific activity in the gas phase. This makes biochemical gas monitoring difficult. We used a micro flow-cell with a FALDH immobilized membrane to prevent the FALDH from deactivation. An ultraviolet light emitting diode (UV-LED) with peak emission of 335nm was employed as an excitation light source. Emission of the UV-LED was introduced to the optode through an optical fiber and fluorescence of NADH was picked up coaxially at the optode. In order to improve the sensitivity, a photomultiplier tube was utilized as a photodetector. Consequently, continuous FA monitoring with biochemical method was successfully conducted with high sensitivity and high selectivity. A real-sample test was also carried out with the bio-sniffer. According to the results, it is expected to be useful in fast and convenient monitoring of indoor FA.
Keywords :
biosensors; fibre optic sensors; gas sensors; light emitting diodes; photodetectors; FALDH immobilized membrane; FALDH reaction; NADH-fluorometric biochemical gas sensor; UV-LED emission; biochemical gas monitoring; biochemical method; biosniffer; continuous FA monitoring; fiber-optic biochemical gas sensor; formaldehyde dehydrogenase reaction; formaldehyde vapor measurement; gas phase; indoor FA monitoring; indoor air quality assessment; indoor formaldehyde assessment; microflow-cell; optical fiber; photodetector; photomultiplier tube; real-sample test; reduced NADH fluorescence; reduced nicotinamide adenine dinucleotide fluorescence; size 335 nm; ultraviolet light emitting diode emission; Band pass filters; Biochemistry; Fluorescence; Gas detectors; Monitoring; Optical fibers; biosensor; enzyme; formaldehyde; gas sensor;
Conference_Titel :
Sensors, 2012 IEEE
Conference_Location :
Taipei
Print_ISBN :
978-1-4577-1766-6
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2012.6411132