Optofluidic glucose sensor utilizing an epoxy-based, transparent dry film resist

An enzymatic, fluorescent glucose sensor was constructed with a novel epoxy-based, optically-transparent dry film resist (PerMX 3000 series, DuPont). This material was utilized as the main active material for fluidic structure and optical interrogation. This sensor was based on the enzymatic reaction of glucose oxidase (GOD) and utilized a ruthenium complex fluorophore as the reporting agent. Both the oxygen-sensitive ruthenium complex and GOD were incorporated into poly(ethylene glycol) (PEG)-based hydrogel materials, which were lithographically patterned and embedded inside the PerMX fluidic channel after wafer-bonding process. The optofluidic sensing platform with this embedded sensing elements exhibited a wide dynamic range of 0-20 mM (0-360 mg/dl) glucose with good sensitivity. This finding demonstrates the promise of PerMX-based fluidic systems as platforms for various optofluidic assays.