Synthesis and fluorescent properties of CdSe quantum dots for the detection of single cells array

As novel quantum fluorescent materials, QDs have longer term photo-stability, narrower emission range, stronger intensity, and broader UV excitation spectra than that of normal fluorescent materials. Due to quantum dots´ (QD) quantum scale effect, QDs of different sizes can emit fluorescence of different colors with the same excitation light, making them suitable bio-probes in multiplexed detection, by which we can achieve multiplexed analysis of cells´ behaviors in real time. In this paper, we first synthesized CdSe QDs in ODE and TOPO system. To enlarge the fluorescent range and get more fluorescent colors, we utilized paraffin liquid as the solvent instead of QDE and oleic acid (OA) as the stabilizer instead of TOPO. Finally, we obtained CdSe QDs emitting colors varying from purple to red in a simple and green experimental condition, reaching the requirement of multiplexed detection. By this method, we synthesized high-quality QD probes for multi-analysis which is low cost, less contaminating and less dependent on equipments.