Fiber-optic endomicroscopy system for high-resolution nonlinear imaging of biological tissue

Author

Wu, Yicong ; Xi, Jiefeng ; Cobb, Michael J. ; Xingde Li

Author_Institution

Department of Bioengineering, University of Washington, Seattle, 98195, USA

fYear

2008

fDate

20-25 Aug. 2008

Firstpage

1851

Lastpage

1852

Abstract

A flexible fiber-optic endomicroscopy system was developed for nonlinear optical imaging of biological samples. Double-clad fiber and photonic bandgap fiber were employed in the endomicroscope for femtosecond pulse delivery, dispersion compensation, nonlinear optical signals collection and fast beam scanning. Three-dimensional imaging of biological tissues based on second harmonic generation and two-photon excited fluorescence was performed with the endomicroscope. The depth-resolved imaging results strongly suggest the potential of this flexible fiber-optic microscope system for real-time assessment of both epithelial and stromal structures in luminal organs.

Keywords

Biological tissues; Biomedical optical imaging; Fiber nonlinear optics; Fluorescence; High-resolution imaging; Optical harmonic generation; Optical imaging; Optical pulses; Photonic bandgap fibers; Ultrafast optics; Animals; Biomedical Engineering; Endoscopes; Imaging, Three-Dimensional; Microscopy; Nonlinear Dynamics; Optical Fibers; Rats; Signal Processing, Computer-Assisted; Swine;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2008. EMBS 2008. 30th Annual International Conference of the IEEE

Conference_Location

Vancouver, BC

ISSN

1557-170X

Print_ISBN

978-1-4244-1814-5

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2008.4649541

Filename

4649541