Title :
Study on Reversibility of Genetic Toxicity of TDI in Mice
Author :
Ji, Yu-bin ; Yu, Miao ; Yu, Lei ; Yang, Wen ; Yang, Ying-jie
Author_Institution :
Center of Res. on Life Sci. & Environ. Sci., Harbin Univ. of Commerce, Harbin, China
Abstract :
This article studied whether genetic toxicity of toluene diisocyanate was reversible. Kunming mice were exposed to TDI by respiratory tract for 14 days in 1/8LC50, 1/4LC50 and 1/2 LC50 dosage and then were feed normally for 14 days. The chromosomal aberrations and the content of RNA & DNA were detected by optical microscope and laser scanning confocal microscope. In 1/4LC50 and 1/2 LC50 group, chromosome aberration rate and the RNA / DNA ratio of TDI were higher than control group significantly (P<;0.01). In addition there was no significant difference between 1/4LC50 and 1/2 LC50 (p> 0.05). The results showed that the damage of TDI on chromosomes and DNA was repairable, but can not be repaired completely.
Keywords :
DNA; cellular biophysics; genetics; optical microscopy; toxicology; DNA; Kunming mice; RNA; chromosomal aberration; genetic toxicity; laser scanning confocal microscopy; optical microscopy; respiratory tract; reversibility; toluene diisocyanate; Biological cells; Biomedical optical imaging; Cells (biology); DNA; Fluorescence; Genetics; Mice; Optical microscopy; RNA; Sealing materials;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5515186
