Cytotoxicity Effects of Nano-Fe3O4 on HeLa Cells

In order to study the cytotoxicity effects of Nano-Fe₃O₄ Particles on HeLa Cells, 50μg/mL, 100μg/mL and 200μg/mL concentrations of Nano-Fe₃O₄ were exposed to HeLa cells, twelve hours later, single cell gel electrophoresis (SCGE) was used to detect HeLa cell DNA molecule damage, the content of the malondialdehyde (MDA) were determined by thiobarbiturie acid reactive substance method and the activity of super oxide dismutase (SOD) was determined by xanthine oxidase method. The results shown that under the 50μg/mL Nano-Fe₃O₄ exposure, compared with the control group, neither the tail moment nor the tail DNA% increased (P>0.05), the increase content of MDA also not significant (P>0.05), but the reduce activity of SOD is significant (P<;0.05); under the 100μg/mL and 200μg/mL Nano-Fe₃O₄ exposure, compared with the control group, the increase of tail moment and tail DNA% both significant (P<;0.05 or P<;0.01), the increase content of MDA also significant (P<;0.05 or P<;0.01), the reduce activity of SOD is significant (P<;0.01). The results suggest that Nano-Fe₃O₄ could induce the cytotoxicity on HeLa cells.