Title :
Improved Transfection Efficiency by the use of Lipofectamine-Modified Plasmid DNA in Laser-Induced Stress Wave-Assisted Gene Transfer: In Vitro Study
Author :
Terakawa, Mitsuhiro ; Otsuka, Risa ; Sato, Shunichi ; Satoh, Yasushi ; Takishima, Kunio ; Ashida, Hiroshi ; Okano, Hideyuki ; Obara, Minoru
Author_Institution :
Dept. of Electron. & Electr. Eng., Keio Univ., Yokohama
Abstract :
We investigated the effect of chemical-modification of plasmid DNA in gene transfection using laser-induced stress waves. Drastic increase in transfection efficiency was demonstrated in vitro, due to the effect of the plasmid DNA modification
Keywords :
biochemistry; cellular biophysics; drugs; genetics; laser applications in medicine; molecular biophysics; patient treatment; chemical modification effect; gene transfection efficiency; in vitro study; laser-induced stress wave-assisted gene transfer; lipofectamine-modified plasmid DNA; Biomembranes; Cells (biology); Chemical lasers; DNA; Educational institutions; Fluorescence; In vitro; In vivo; Laser theory; Stress;
Conference_Titel :
Lasers and Electro-Optics Society, 2006. LEOS 2006. 19th Annual Meeting of the IEEE
Conference_Location :
Montreal, Que.
Print_ISBN :
0-7803-9555-7
Electronic_ISBN :
0-7803-9555-7
DOI :
10.1109/LEOS.2006.278818
