Isolation of One S. cerevisiae BY-14 Mutant BL-23 with High-Yield Production of Glutathione by Ion Implantation

Author

Guo, Xuewu ; Shi, Liping ; Yang, Hongjiang ; Xiao, Dongguang

Author_Institution

Key Lab. of Ind. Microbiol., Tianjin Univ. of Sci. & Technol., Tianjin, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

3

Abstract

In order to obtain a higher glutathione (GSH) production, the original strain of S. cerevisiae BY-14 was mutated with N+ implantation (10 keV, 60×10¹⁴ ions/cm²) , and an ethionine-resistant mutant BL-23 was obtained. The mutant showed GSH production increased from 58.79 mg/l to 113.46 mg/l, and content increased from 14.17 mg/g dry cell weight to 20.86 mg/g dry cell weight. The mutant BL-23 is stable in genetic. Its higher glutathione producing ability could be inherited after ten times subcultures.

Keywords

biochemistry; cellular biophysics; ion implantation; microorganisms; molecular biophysics; nitrogen; BY-14; N; S. cerevisiae; dry cell weight; electron volt energy 10 keV; ethionine-resistant mutant BL-23; glutathione; high-yield production; ion implantation; mass 113.46 mg; mass 14.17 mg; mass 20.86 mg; mass 58.79 mg; strain; Capacitive sensors; Fungi; Genetics; Ion beams; Ion implantation; Microorganisms; Nitrogen; Plasma temperature; Production; Sugar;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5517073

Filename

5517073