Purification and Identification of Soluble Catechol-O-Methyltransferase from Rat Liver

Author

Yang, Lina ; Yan, Jianhui ; Deng, Yulin

Author_Institution

Sch. of Life Sci. & Technol., Beijing Inst. of Technol., Beijing, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

4

Abstract

Soluble catechol-O-methyltransferase (S-COMT) is an important target protein due to its involvement in some human disorders. The main scope of the work was to develop an approach to purify and identify S-COMT. Its isoelectric point was 5.0 determined with Offgel. A simplified process including extraction, selective precipitation, ammonium sulfate precipitation, gel filtration chromatography and anion exchange chromatography was established to obtain S-COMT of a certain purity degree. The molecular was 25 kD. Mass spectrometry and mascot were employed for further identification.

Keywords

biochemistry; chromatography; enzymes; ion exchange; liver; mass spectroscopy; precipitation (physical chemistry); proteomics; S-COMT; ammonium sulfate precipitation; anion exchange chromatography; extraction; gel filtration chromatography; human disorders; isoelectric point; liver; purification; rat; selective precipitation; soluble catechol-o-methyltransferase; target protein; Biomembranes; Chemical analysis; Filtration; Liver diseases; Mass spectroscopy; Parkinson´s disease; Pharmaceutical technology; Proteins; Purification; Strips;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5517422

Filename

5517422