Iso-thermal DNA reactions and assays in microfabricated capillary electrophoresis systems

Author

Tang, Thompson ; Ocvirk, Gregor ; Harrison, D. Jed

Author_Institution

Dept. of Chem., Alberta Univ., Edmonton, Alta., Canada

Volume

1

fYear

1997

fDate

16-19 Jun 1997

Firstpage

523

Abstract

The combination of microfluidic structures and electrokinetic separation and pumping on a glass chip, provides the capability for fast integrated, and more highly automated DNA assays. The Cycling Probe Technology reaction (CPT), used for signal enhancement of target DNA is an ideal candidate for integration on-chip. We show the application of the chip format for separations of CPT reaction products. Sensitivity with the chip format can be enhanced using epifluorescence confocal detection

Keywords

DNA; biomedical equipment; biosensors; biotechnology; electrophoresis; micropumps; microsensors; separation; calibration curve; chip format; cycling probe technology reaction; electrokinetic separation; epifluorescence confocal detection; fast integrated assays; glass chip; highly automated DNA assays; hybridization assays; isothermal DNA reactions; microfabricated capillary electrophoresis systems; microfluidic structures; pumping; reaction products separation; signal enhancement; target DNA; Annealing; Biochemistry; Chemical technology; Chemistry; DNA; Electrokinetics; Glass; Microfluidics; Probes; Temperature;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on

Conference_Location

Chicago, IL

Print_ISBN

0-7803-3829-4

Type

conf

DOI

10.1109/SENSOR.1997.613702

Filename

613702