DocumentCode :
3142191
Title :
Degradation of Hemicelluloses of Cottonseed Coat by Xylanase
Author :
Hu, Ying-qing ; Nie, Hua-Li ; Zhu, Li-Min ; Branford-White, Christopher J.
Author_Institution :
Coll. of Chem., Chem. Eng. & Biotechnol., Donghua Univ., Shanghai, China
fYear :
2010
fDate :
18-20 June 2010
Firstpage :
1
Lastpage :
4
Abstract :
The hemicelluloses were separated from the cottonseed coat and degraded with Pulpzyme HC. The digestion degree value was calculated by the equation depended on the release of reducing sugars concentration which was determined from DNS fitting curve equation. The optimum enzyme reaction temperature was 60°C and pH was 5.5. The duration of 80 min was chose as the most suitable enzymolysis time. The degradation degree value was 57.5% when 0.24 ml pure Pulpzyme HC was added with one milligramme hemicelluloses powder. The K+ content substrate (0.5 mmol L-1) enhanced the enzymic degradation of hemicelluloses. The other metal ions were found to be some inhibitors of Pulpzyme HC. The effects of chelating agent (EDTA) were complex and no trend was observed.
Keywords :
cotton; enzymes; textile industry; waste management; DNS fitting curve equation; cottonseed coat; digestion degree value; enzymic degradation; enzymolysis time; hemicelluloses; metal ions; pulpzyme HC; sugars concentration; xylanase; Biochemistry; Bleaching; Chemicals; Cotton; Degradation; Equations; Impurities; Powders; Temperature; Textiles;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
ISSN :
2151-7614
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
Type :
conf
DOI :
10.1109/ICBBE.2010.5517547
Filename :
5517547
Link To Document :
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