DocumentCode :
3146274
Title :
Adsorption-induced inactivation of heavy meromyosin on polymer surfaces imposes effective drag force on sliding actin filaments in vitro
Author :
Hanson, Kristi L. ; Solana, Gerardin ; Vaidyanathan, Viswanathan ; Nicolau, Dan V.
Author_Institution :
Fac. of Eng. & Ind. Sci., Swinburne Univ. of Technol., Hawthorn, Vic.
fYear :
2006
fDate :
9-12 May 2006
Firstpage :
151
Lastpage :
154
Abstract :
Actin and myosin are of interest as potential force-generating elements in engineered nanodevices. Such applications require surface coatings which are both biocompatible and amenable to nanolithographic processing, but the manner in which surfaces modulate motor protein function has not been rigorously studied. Here we examine motor protein surface density and bioactivity on a variety of polymer surfaces, and compare the results to in vitro actomyosin motility characteristics. Filament velocities were found to be controlled by the proportion, rather than density, of active heavy meromyosin (HMM), consistent with the imposition of an effective drag force by inactivated HMM due to weak actin-binding interactions. Interpretation of the results with respect to previous models suggests that the inactive HMM fraction has no force-generating ability, and that the effective drag imposed on polystyrene is lower than that on methacrylate polymers and nitrocellulose, consistent with a higher degree of protein denaturation on aromatic surface structures
Keywords :
adsorption; microbalances; molecular biophysics; nanolithography; polymer films; proteins; actin-binding interaction; active heavy meromyosin; actomyosin motility characteristics; adsorption-induced inactivation; aromatic surface structure; bioactivity; drag force; engineered nanodevices; force-generating elements; motor protein function; nanolithographic processing; nitrocellulose; polymer surface coating; protein denaturation; quartz crystal microbalance; sliding actin filaments; surface density; Coatings; Drag; Force control; Hidden Markov models; In vitro; Micromotors; Polymers; Proportional control; Protein engineering; Velocity control; Actomyosin mechanics; in vitro motility assay; polymer surface coatings; protein adsorption; quartz crystal microbalance;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Microtechnologies in Medicine and Biology, 2006 International Conference on
Conference_Location :
Okinawa
Print_ISBN :
1-4244-0338-3
Electronic_ISBN :
1-4244-0338-3
Type :
conf
DOI :
10.1109/MMB.2006.251514
Filename :
4281332
Link To Document :
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