Title :
High-throughput cell-based screening system with on-chip dilution stage
Author :
Greve, F. ; Seemann, L. ; Bonneick, S. ; Lichtenberg, J. ; Hierleman, A.
Author_Institution :
Phys. Electron. Lab., Eidgenossische Tech. Hochschule, Zurich
Abstract :
A microchip-based approach for performing a complete and automated drug-screening assay on living cells is presented. Cells are trapped and immobilized in a small 0.5-mul-volume incubation chamber by means of orifice microstructures and are subsequently incubated with drug dilutions ranging over three orders of magnitude. The microsystem includes a perforated silicon chip embedded in a larger elastomer substrate, which features the microfluidic network and the incubation chamber. This article describes the modeling and the fabrication of the microchip components, immobilization of normal human dermal fibroblasts (NHDFs) and a screening experiment with cultured NHDFs, which have been exposed to a fluorescent cell tracker
Keywords :
bioMEMS; biological techniques; cellular biophysics; drugs; elastomers; lab-on-a-chip; microfluidics; automated drug-screening assay; cell culture; cell immobilization; cell trapping; drug dilutions; elastomer substrate; fluorescent cell tracker; high-throughput cell-based screening system; incubation chamber; microchip component fabrication; microchip component modeling; microchip-based approach; microfluidic network; microsystem; normal human dermal fibroblasts immobilization; on-chip dilution stage; orifice microstructures; perforated silicon chip; Dermis; Drugs; Fabrication; Fibroblasts; Humans; Microfluidics; Microstructure; Orifices; Silicon; System-on-a-chip; Cell immobilization; dilution stage; drug-screening device; microfluidics;
Conference_Titel :
Microtechnologies in Medicine and Biology, 2006 International Conference on
Conference_Location :
Okinawa
Print_ISBN :
1-4244-0338-3
Electronic_ISBN :
1-4244-0338-3
DOI :
10.1109/MMB.2006.251525