Title :
Analysis of glycosides in traditional chinese medicine using electrochemical detection
Author :
Kwon, Ha-Jeong ; Park, Yong-duk
Author_Institution :
Dept. of Preventive Dentistry, Kyung Hee Univ., Seoul, South Korea
Abstract :
High performance liquid chromatography (HPLC) method was coupled with pulsed amperometric detector, a kind of electrochemical detector by combining with post-column sodium hydroxide solution in order to analyze glycosides. Using this method, the contents of amygdalin, baicalin, naringin, and hesperidin in TCMs were determined with isocratic elution of 13% acetonitrile with 10 mM sodium phosphate buffer (pH 6.9). The coefficient of linear regression was 0.9997-0.9999 for concentrations between 1 and 100 μg/mL. The precision (RSD %) was less than 8.89% in Chong-Phyo-Tang and Run-Chang-Tang. This method showed high selectivity for analyzing glycosides without any interfering peaks. Therefore, glycosides in TCMs were conveniently analyzed without any pretreatment steps.
Keywords :
biomedical engineering; chromatography; electrochemical analysis; molecular biophysics; organic compounds; regression analysis; amygdalin; baicalin; electrochemical detection; glycosides; hesperidin; high performance liquid chromatography; linear regression coefficient; naringin; pulsed amperometric detector; traditional chinese medicine; Compounds; Detectors; Drugs; Electric potential; Quality control; Sensitivity; Sugar; glycosides; pulsed amperometric detection; quality control; traditional Chinese medicine;
Conference_Titel :
Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on
Conference_Location :
Yantai
Print_ISBN :
978-1-4244-6495-1
DOI :
10.1109/BMEI.2010.5640085
