DocumentCode :
3157070
Title :
Anti-Tumor Effect of Newcastle Disease Virus Vaccine for Human Use Preparation Process
Author :
Wan, Xue-qin ; Li, Hong-ming ; Zhan, Bing-tuan ; Li, Wan-kelan
Author_Institution :
Med. Coll., Foshan Univ., Foshan, China
fYear :
2010
fDate :
18-20 June 2010
Firstpage :
1
Lastpage :
3
Abstract :
This project focuses on the improvement of NDV production technology and innovation of the NDV vaccine preparation process with cell culture technology. Methods: To use primary chick embryo cell culture system in preparation of NDV anti-tumor vaccine. Results:In the new vaccine development process, the focused on maintaining and improving NDV titer while reducing the residual bovine serum. So that the new vaccine achieve the requirements of national´s human biological products standards. 1. Using a small number of high-quality calf serum (2%) before addition of NDV to the cells and washing single cell layer with serum-free medium after the confluence of the cell to reduce the content calf serum. 2. Replacing calf serum to human albumin in addition of NDV. The residual of bovine serum protein contents is lower than 50 ng/ml (reverse indirect hemagglutination assay) after using of these methods. NDV Outcome culture fluid Log PFU≥5, 5, hemagglutinin titer 1:640.Conclusions:The potency and activity of NDV have reached the quality requirements of biological products in China.
Keywords :
biochemistry; cellular biophysics; diseases; microorganisms; molecular biophysics; proteins; tumours; NDV activity; NDV potency; NDV production technology; NDV vaccine preparation process; Newcastle disease virus vaccine; antitumor effect; bovine serum protein; calf serum; cell culture technology; culture fluid Log; hemagglutination assay; hemagglutinin titer; high-quality calf serum; human albumin; human use preparation process; primary chick embryo cell culture system; residual bovine serum; serum-free medium; vaccine development process; Biomedical engineering; Bovine; Dairy products; Diseases; Embryo; Humans; Protein engineering; Testing; Tumors; Vaccines;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
ISSN :
2151-7614
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
Type :
conf
DOI :
10.1109/ICBBE.2010.5518276
Filename :
5518276
Link To Document :
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