Identification of epitopes in Echinococcosis multilocularis 14-3-3 antigen: A bioinformatics approach

Em14-3-3 protein is reported as the important antigen in the immunology of Echinococcosis multilocularis. The sequence of Em14-3-3(Accession No: AF529419) from NCBI databank has been taken for this study. Computer-based prediction program results show that the B cell epitopes in the alleles of: 11-34,38-53,61-70,74-84,91-100,114-122,134-158,189-224, 229-244. The different structural features of the protein have also been predicted including glycosylation, kinase C phosphorylation, casein kinase II phosphorylation and N-myristylation sites. These computational prediction programs show four glycosylation, five kinase C phosphorylation, two casein kinase II phosphorylation, zero N-myristylation sites and seven disulphide sites. Development and approval of new vaccines are the keys for control of AE.