Microfabrication of a flow immunoassay for the detection of aflatoxin

Author

Rabbany, Sina Y. ; Wong, Eddy Y. ; Sheppard, Norman F., Jr.

Author_Institution

Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA

Volume

1

fYear

1996

fDate

31 Oct-3 Nov 1996

Firstpage

260

Abstract

A flow-displacement immunoassay has been implemented by microfabricating a reactor in a silicon wafer. To increase surface area, antibodies immobilized on a porous support matrix have been introduced into the reactor. To characterize the dissociation kinetics of aflatoxin (AFB₂) from the affinity-resin loaded reactor, experiments were conducted at flow rates of 0.15, 0.5, 1.0. and 2.0 ml/min. Dissociation curves, measured using the intrinsic fluorescence of AFB ₂, showed an exponential decrease in dissociated AFB₂ over time. The apparent dissociation rate constants (k_d) values were shown to be flow rate dependent. The k_d was extrapolated to be 2.30×10^-4 sec^-1 at infinite flow rate

Keywords

biosensors; chemical sensors; dissociation; micromachining; microsensors; reaction rate constants; Si; affinity-resin loaded reactor; aflatoxin detection; antigen-antibody binding; apparent dissociation rate constants; dissociation curves; dissociation kinetics; flow rate dependent; flow-displacement immunoassay; immobilized antibodies; immunosensors; infinite flow rate; intrinsic fluorescence; microfabrication; micromachined reactors; porous support matrix; reactor fabrication; silicon wafer; Biomedical engineering; Chemical analysis; Electronic mail; Engineering in Medicine and Biology Society; Fluorescence; Immune system; Inductors; Kinetic theory; Resists; Silicon;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1996. Bridging Disciplines for Biomedicine. Proceedings of the 18th Annual International Conference of the IEEE

Conference_Location

Amsterdam

Print_ISBN

0-7803-3811-1

Type

conf

DOI

10.1109/IEMBS.1996.656943

Filename

656943