Combining Raman scattering technique with dielectrophoresis chip for clinical isolates Helicobacter pylori analysis

A rapid trapping and detection method for clinical isolates Helicobacter pylori (H. pylori) analysis is reported in this study. Different strain of H. pylori are trapped in 3 dimension dielectrophoresis chip (DEP) under the condition of 20 mM PBS, voltage at 20 Vp-p and frequency at 300 kHz. H pylori #238(Wild type) presents more detail peaks in Raman spectra than that of H. pylori #603(gene knockout). In clinical isolates analysis, significant difference between Duodenal Ulcer (DU, n=5) and Gastric Cancer (GC, n=5) are observed and showed the 70% and 80% of correction rate obtained from the mathematical results by PCA and HCA analysis. Clinical isolates H. pylori can be identified directly via this technique within 3 min.