In vitro culture human mesenchymal stem cells with a novel BMP combined porous composite

Novel porous BG-COL-HYA-PS bioactive composites composed of a sol-gel processed bioactive glass (BG), collagen (COL), hyaluronic acid (HYA) and phosphatidylserine (PS) have been developed for bone tissue engineering applications. In this study, the in vitro performance of the porous composites with or without combining bone morphogenetic protein (BMP) was evaluated after culture with human mesenchymal stem cells (hMSCs) by: (1) phase contrast microscopy for cell morphology after culture with the composites; (2) MTT assay for cell viability and proliferation, and (3) alkaline phosphatase (ALP) and osteocalcin (OC) assays for cell differentiation after culture in extracts from the composites at 1, 3 and 7 d. The results showed that cells attached and spread on and around the composites after 1 d. The cells proliferated during the culture period. The composites are not cytotoxic and are compatible with hMSCs after 7 d. Furthermore, the cellular ALP activity and OC concentration for the culture with BMP-combined composites are significantly higher than those for the composites alone and control. To investigate the influence of cell seeding density on the distribution and growth of human bone marrow stem cells (BMSCs) on the composite . Human BMSCs were labeled with Dil and seeded on the composite. The adhesion efficiency was assessed.