Rotational positioning method based on the microscopic vision

This paper reported a rotational positioning method based on the microscopic vision. Rotational positioning method has important applications in image scanning of biological tissue or cell injection experiments. A compact rotational stage driven by a stepper motor is utilized, and cross mark alignment method with unequal length is proposed to aid positioning. Feature tracking algorithm is utilized to track the target in real time. Combined with the X/Y stage of the inverted microscope and image-based visual servo controller, positioning the biological sample could be completed. The experiment shows that, after positioning the two cross marks´ angle deviation is 0.462° and the position difference is 2~3 μm meeting with the biological operation requirements.