Title :
Using impedance biosensors to distinguish the PC12 cells adhesion and differentiation
Author :
Chen, Chen-Chia ; Lin, Shan-Yu ; Sheu, Jeng-Tzong
Author_Institution :
Dept. of Mater. Sci. & Eng., Nat. Chiao Tung Univ., Hsinchu, Taiwan
Abstract :
Some properties of living cells are associated with cell adhesion and cell spreading process. Traditional way to monitor cells´ morphological changes is usually the use of optical microscope. Biosensors provide an alternative to automatically monitor cell adhesion and spreading process. This study utilized microelectrodes array based impedance biosensors to record cells attachment and differentiation via changes of impedance. The results were confirmed with the images of optical microscope. Based on the electrical model proposed, cell adhesion and differentiation can be clearly identified without any additional biochemical tests.
Keywords :
adhesion; amperometric sensors; biomechanics; biosensors; cellular biophysics; PC12 cell adhesion; PC12 cell differentiation; cell attachment; electrical model; microelectrodes array based impedance biosensors; optical microscopy; Adhesives; Biosensors; Cells (biology); Electrodes; Gold; Immune system; Impedance;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on
Conference_Location :
Kaohsiung
Print_ISBN :
978-1-61284-775-7
DOI :
10.1109/NEMS.2011.6017478
