Title :
Optimization of nanostructured metal layers for DNA hybridization monitoring in a SPR-i experiment
Author :
Manera, M.G. ; Rella, R. ; Spadavecchia, J. ; Moreau, J. ; Canva, M. ; Savchenko, A.
Author_Institution :
Inst. for Microelectron. & Microsyst., IMM-CNR Lecce, Lecce, Italy
Abstract :
The critical influence of the structural and morphological properties of a thin gold metal layer deposited on glass substrates on the surface chemistry binding efficiency in surface plasmon resonance-imaging biochip systems is demonstrated in this work. Interesting variations of DNA-DNA hybridization signal is observed by using Au substrates annealed at different temperatures.
Keywords :
DNA; X-ray diffraction; annealing; atomic force microscopy; biochemistry; biosensors; gold; lab-on-a-chip; metallic thin films; molecular biophysics; nanobiotechnology; nanostructured materials; optical sensors; scanning electron microscopy; surface chemistry; surface plasmon resonance; titanium; DNA hybridization monitoring; DNA probe immobilization; DNA-DNA hybridization signal; SiO2; Ti-Au; X-ray diffraction; annealing; atomic force microscopy; biochip systems; functionalization chemistries; glass substrates; morphological properties; nanostructured metal layers; plasmon resonance transducer; scanning electron microscopy; structural properties; surface chemistry binding efficiency; surface plasmon resonance imaging; thin gold metal layer; Annealing; Chemistry; DNA; Glass; Gold; Monitoring; Plasmons; Resonance; Surface morphology; Temperature;
Conference_Titel :
Sensors, 2009 IEEE
Conference_Location :
Christchurch
Print_ISBN :
978-1-4244-4548-6
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2009.5398368
