DocumentCode :
3284240
Title :
A novel system for physiologic pulsatile perfusion of intact vascular tissue
Author :
Conklin, B. ; Surowiec, S. ; Lin, P. ; Chen, C.
Author_Institution :
Dept. of Mech. Eng., Georgia Inst. of Technol., Atlanta, GA, USA
Volume :
1
fYear :
1999
fDate :
1999
Abstract :
A novel system has been designed and tested for perfusion culture of intact vascular tissue. The system uses a cam-driven syringe to impose the pulsatile component of flow on the steady flow generated by a peristaltic pump and compliance chamber. The system is capable of producing flow waveforms that include back-flow phases as seen in the femoral arteries of humans. Three physiologic waveforms including canine femoral and carotid and human left coronary artery waveforms have been generated. The system also allows independent control of flow-rate and pressure. Porcine common carotid and left coronary arteries, human greater saphenous veins, and rhesus macaque femoral and common carotid arteries have been cultured in the system for up to 48 hours. Viability of vascular segments after culture was assessed by a contraction and relaxation assay which demonstrates both smooth muscle contractility and endothelial cell mediated smooth muscle relaxation
Keywords :
biological techniques; blood vessels; cellular biophysics; haemorheology; muscle; pulsatile flow; 48 h; back-flow phases; cam-driven syringe; common carotid arteries; contraction/relaxation assay; cultured vascular segments viability; endothelial cell mediated smooth muscle relaxation; flow waveforms; human greater saphenous veins; intact vascular tissue; left coronary arteries; physiologic pulsatile perfusion; porcine common carotid artery; rhesus macaque femoral arteries; smooth muscle contractility; steady flow; Arteries; Biological system modeling; Cells (biology); Control systems; Hemodynamics; Humans; Muscles; Pressure control; System testing; Veins;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
ISSN :
1094-687X
Print_ISBN :
0-7803-5674-8
Type :
conf
DOI :
10.1109/IEMBS.1999.802163
Filename :
802163
Link To Document :
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